Microorganisms such as bacteria and fungi play essential roles in many application fields, like biotechnique, medical technique and industrial domain. Microorganism counting techniques are crucial in microorganism analysis, helping biologists and related researchers quantitatively analyze the microorganisms and calculate their characteristics, such as biomass concentration and biological activity. However, traditional microorganism manual counting methods, such as plate counting method, hemocytometry and turbidimetry, are time-consuming, subjective and need complex operations, which are difficult to be applied in large-scale applications. In order to improve this situation, image analysis is applied for microorganism counting since the 1980s, which consists of digital image processing, image segmentation, image classification and suchlike. Image analysis-based microorganism counting methods are efficient comparing with traditional plate counting methods. In this article, we have studied the development of microorganism counting methods using digital image analysis. Firstly, the microorganisms are grouped as bacteria and other microorganisms. Then, the related articles are summarized based on image segmentation methods. Each part of the article is reviewed by methodologies. Moreover, commonly used image processing methods for microorganism counting are summarized and analyzed to find common technological points. More than 144 papers are outlined in this article. In conclusion, this paper provides new ideas for the future development trend of microorganism counting, and provides systematic suggestions for implementing integrated microorganism counting systems in the future. Researchers in other fields can refer to the techniques analyzed in this paper.
Russian Journal of Nondestructive Testing - Nondestructive testing and evaluation technology of the total focusing method (TFM) based on full matrix capture (FMC) is a hot spot in the current array... 相似文献
Mine Water and the Environment - Dysart Woods is the largest (≈ 23 ha) old-growth forest in southeastern Ohio. It was donated to Ohio University for research purpose and public... 相似文献
A foil–microchannel plate(MCP) detector,which uses electrostatic lenses and possesses both good position and timing resolutions, has been designed and simulated for beam diagnostics and mass measurements at the next-generation heavy-ion-beam facility HIAF in China. Characterized by low energy loss and good performances of timing and position measurements, it would be located at focal planes in fragment separator HFRS for position monitoring, beam turning, Bρ measurement, and trajectory reconstruction. Moreover, it will benefit the building-up of a magnetic-rigidity–energy-loss–time-offlight(Bρ-△E-TOF) method at HFRS for high-precision in-flight particle identification of radioactive isotope beams on an event-by-event basis. Most importantly, the detector can be utilized for in-ring TOF and position measurements,beam-line TOF measurements at two achromatic foci, and position measurements at a dispersive focus of HFRS, thus making it possible to use two complementary mass measurement methods [isochronous mass spectrometry at the storage ring SRing and magnetic-rigidity–time-of-flight(Bρ-TOF) at the beam-line HFRS] in one single experimental run. 相似文献
It is well known that the finite element method (FEM) encounters dispersion errors in coping with mid-frequency acoustic problems due to its “overly stiff” nature. By introducing the generalized gradient smoothing technique and the idea of condensed shape functions with virtual nodes, a cell-based smoothed radial point interpolation method is proposed to solve the Helmholtz equation for the purpose of reducing dispersion errors. With the properly selected virtual nodes, the proposed method can provide a close-to-exact stiffness of continuum, leading to a conspicuous decrease in dispersion errors and a significant improvement in accuracy. Numerical examples are examined using the present method by comparing with both the traditional FEM using four-node tetrahedral elements (FEM-T4) and the FEM model using eight-node hexahedral elements with modified integration rules (MIR-H8). The present cell-based smoothed radial point interpolation method has been demonstrated to possess a number of superiorities, including the automatically generated tetrahedral background mesh, high computational efficiency, and insensitivity to mesh distortion, which make the method a good potential for practical analysis of acoustic problems. 相似文献
正At the instigation of some of Emeritus Professor Edwin Thomas(Ted) Brown’s PhD students, a Colloquium in his honour to celebrate his retirement and a long and distinguished career in rock mechanics was held in Brisbane, Australia on the 4th December2018, Ted’s 80th birthday.Ted was born in Castlemaine, Victoria, Australia, on 4 December 相似文献